Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 23)

Section 6
Programming a New Method
In the NEW METHOD mode a method containing one (1) to ten (10) steps can be
programmed. Each method allows programming of the following parameters:
A rehydration step (optional)
•
passive or active at 50 V
• rehydration temperature (10–25 °C)
• rehydration time up to 99:00 hours
A current limit up to 99 µA per strip. 50 µA per strip is recommended to prevent IPG
strip overheating.
The isoelectric focusing temperature (10–25 °C)
Ten separate steps each programmable for:
•
Voltage
• Voltage ramping slope (rapid, linear, or slow)
• Time in hours:minutes or volt-hours.
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