Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 11)

Casting Stand The casting stand holds the gel sandwich upright
while casting a gel (Figure 3.5). With the cam levers
engaged, the sponge seals the bottom of the gel
while the acrylamide polymerizes.
Fig. 3.5. Casting Stand.
Sandwich Clamps The sandwich clamps consist of a single screw
mechanism which makes assembly, alignment, and
disassembly of the gel sandwich an effortless task.
The clamps exert an even pressure over the entire
length of the glass plates. A set consists of a left and
right clamp.
Alignment Card The alignment card simplifies sandwich assembly by
keeping the spacers in the correct position.
Comb Gasket Holder The comb gasket holder holds the comb gasket that
prevents (DGGE only) leakage of acrylamide during gel
casting (Figure 3.6). The front of the holder has two
screws which are used to secure the comb gasket
against the glass plate. The top of the comb gasket
holder also has two tilt rod screws which control the
position of the tilt rod during gel casting. The opposite
side of the comb gasket holder has two vent ports. There
are two sizes of comb gasket holder: a 1 mm size for
1 mm and 0.75 mm spacer sets and a 1.5 mm size for
the 1.5 mm spacer set.
Fig. 3.6. Comb gasket holder.
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Notched step
Gasket holder
Comb gasket screws
Tilt rod