Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 14)

Model 475 System Components
Description (DGGE System only)
Syringe Two pairs each of 10 and 30 ml disposable syringes are
provided. The 10 ml syringes are for gel volumes less than
10 ml. The 30 ml syringes are for gel volumes greater than
10 ml. For proper gel casting, use matching syringe sizes.
Plunger Caps/ There are two plunger caps, one for each syringe. The
Plunger Cap Screw plunger caps fit both the 10 and 30 ml syringes (Figure 3.11).
Lever Attachment Screw The lever attachment screw is on the plunger cap. This
screw fits into the groove of the lever and conducts the driving
force of the cam in dispensing the gel solution.
Syringe Sleeve One pair of syringe sleeves for each size syringe is
provided (Figure 3.12). The sleeve is a movable adaptor to
mount the syringe in the holder. The sleeve should conform to
the syringe. If the syringe is too tight or too loose, adjust the
sleeve by pushing or pulling.
Syringe Holder/ The syringe holder is next to the lever. It holds the syringe
Syringe Holder Screw in place and controls the delivery volume. The syringe is held
in the holder by tightening the holder screw against the sleeve.
Volume Adjustment Screw The volume adjustment screw is on both sides of the syringe
holder (Figure 3.10). It adjusts the holder to the desired delivery
volume.
Volume Setting Indicator The volume setting indicator is at the top corner of the syringe
holder nearest the volume setting numbers (Figure 3.10).
Lever The position of the lever is controlled by the rotation of the
cam (Figure 3.10). The lever must be in the vertical or start
position before use.
Tygon Tubing One length of Tygon ™ tubing is provided. Cut the tubing into two
15.5 cm and one 9 cm lengths. The longer pieces are used to
transport the gel solution from the syringes into the Y-fitting. The
short piece will transport the gel solution from the Y-fitting to
the gel sandwich.
Y-Fitting The Y-fitting mixes the high and low density gel
solutions (Figure 3.13).
Luer Fitting/Coupling There are two luer fittings that fit both 10 and 30 ml syringes.
The fittings twist onto the syringe and connect to the Tygon
tubing on the other end. A luer coupling is used on one end
of the 9 cm tubing to connect it to the gel sandwich stopcock.
10
Fig. 3.11. Plunger Cap Fig. 3.12. Syringe sleeve Fig. 3.13. Y-Fitting
Lever
attachment
screw
Plunger cap
screw