Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 71)

Fig. 7.3. Attaching the clamps to the glass plate assembly.
5. Place the sandwich assembly in the alignment slot (the slot without cams) of the casting
stand with the short glass plate forward (Figure 7.4). Loosen the sandwich clamps and
insert an alignment card to keep the spacers parallel to the clamps.
Note: Always use the alignment slot and alignment card to set the spacers in place. Failure
to use these can result in gel leakage when casting, as well as buffer leakage during the
run.
6. Align the plates and spacers by simultaneously pushing inward on both clamps at the locating
arrows while pushing down on the spacers with your thumbs. Tighten both clamps just enough
to hold the sandwich in place. Pushing inward on both clamps at the locating arrows will insure
that the spacers and glass plates are flush against the sides of the clamps (Figure 7.4).
Fig. 7.4. Aligning spacers in the sandwich assembly.
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