Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 12)

Stopcocks (DGGE only) The gel solution is introduced via the stopcock at the
inlet port on the sandwich clamp when casting a per-
pendicular gel (Figure 3.7).
Fig. 3.7. Stopcock
Comb and Spacer Set The 7.5 x 10 cm gel format consist of two “mirror image
(DGGE only)spacers, one middle spacer and a dual
prep comb for two 7.5 x 10 cm gels (Figure 3.8). The
spacers have a groove and injection port hole for
casting. The middle spacer between the two gels fits
into the middle notch of the dual comb and allows the
air to escape through the comb gasket holder vent port.
The 16 x 16 cm gel format consist of two different
spacers, one with the groove and injection port hole
for casting and one with a short groove toward the
injection port hole for air to escape. A single prep comb
without a middle spacer is provided with the 16 x 16 cm
gel format.
Fig. 3.8. Dual prep comb and spacer set for two 7.5 x 10 cm gels.
Comb and Spacer Set Different types of combs and spacers are provided with
the different DCode systems. Spacer lengths are
10 cm, 16 cm or 20 cm, with a thickness of 0.75 or
1.0 mm. Combs come with 16 or 20 wells and a
thickness of 0.75 or 1.0 mm.
8
Inlet fitting
Injection port holes
groove