Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 70)

7.6 Adding the Running Buffer
1. Remove and place the temperature control module on the DCode lid stand.
2. Add 2 or 7 L of running buffer to the electrophoresis tank.
Note: To improve heat dissipation during electrophoresis, 7 L of buffer can be used.
3. Place the temperature control module on the electrophoresis tank.
7.7 Assembling the PTT Gel Sandwich
For PTT gel formats, a 20 x 20 cm gel sandwich is used. To insure proper alignment,
make sure all plates and spacers are clean and dry before assembly. Use caution when
assembling the glass plate sandwiches. Wear gloves and eye protection at all times.
1. Assemble the gel sandwich on a clean surface. Lay the large rectangular plate down first,
then place the left and right spacers of equal thickness along the short edges of the larger
rectangular plate.
2. Place a short glass plate on top of the spacers so that it is flush with the bottom edge of
the long plate.
3. Loosen the single screw of each sandwich clamp by turning counterclockwise. Place each
clamp by the appropriate side of the gel sandwich with the locating arrows facing up and
toward the glass plates (Figure 7.2).
Fig. 7.2. Positioning glass plates, spacers, and clamps.
4. Grasp the gel sandwich firmly. Guide the left and right clamps onto the sandwich so that
the long and short plates fit the appropriate notches in the clamp (Figure 7.3). Tighten
the screws enough to hold the plates in place.
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