Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 47)

Fig. 4.26. Aligning spacers in the sandwich assembly.
6. Align the plates and spacers by simultaneously pushing inward on both clamps at the
locating arrows while, at the same time, pushing down on the spacers with your thumbs.
Tighten both clamps just enough to hold the sandwich in place. Pushing inward on both
clamps at the locating arrows will insure that the spacers and glass plates are flush against
the sides of the clamps (Figure 4.26).
7. Remove the alignment card. Remove the sandwich assembly from the casting stand and
check that the plates and spacers are flush at the bottom. If they are not flush, realign the
sandwich and spacers to obtain a good seal (Repeat steps 5–7).
8. When a good alignment and seal is obtained, tighten the clamp screws until it is finger-tight.
Casting TTGE Gels
1. Place the gray sponge onto the front casting slot. The camshafts on the casting stand
should have the handles pointing up and pulled out. Place the sandwich assembly on the
sponge with the shorter plate facing forward. When the sandwich is placed correctly, press
down on the sandwich and turn the handles of the camshaft down so that the cams lock
the sandwich in place.
2. Into a 50 ml tube, add the required amount of gel solution (Section 4.3). Add a final
concentration of 0.09% (v/v) each of ammonium persulfate and TEMED solution. Cap
the tube and mix.
3. Insert a comb in the gel sandwich and tilt it so the teeth are at a slight angle. This will prevent
air from being trapped under the comb teeth when pouring the gel solution (Figure 4.27).
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