Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 4)

5.8 Casting Heteroduplex Analysis Gels ........................................................51
Section 6 Single-Stranded Conformational Polymorphism (SSCP) .........52
6.1 Introduction to SSCP ..............................................................................52
6.2 Reagent Preparation ...............................................................................54
6.3 Gel Volumes ............................................................................................56
6.4 Sample Preparation ................................................................................56
6.5 Temperature Controller............................................................................57
6.6 Cooling the Running Buffer and Chiller Settings.......................................58
6.7 Assembling the SSCP Gel Sandwich.......................................................58
6.8 Casting SSCP Gels .................................................................................60
Section 7 Protein Truncation Test (PTT).....................................................61
7.1 Introduction to PTT .................................................................................61
7.2 Reagent Preparation ...............................................................................62
7.3 Gel Volumes ............................................................................................64
7.4 Sample Preparation .................................................................................64
7.5 Temperature Controller............................................................................65
7.6 Adding the Running Buffer .......................................................................66
7.7 Assembling the PTT Gel Sandwich..........................................................66
7.8 Casting PTT Gels ....................................................................................68
Section 8 Electrophoresis ...........................................................................69
8.1 Assembling the Upper Buffer Chamber....................................................69
8.2 Sample Loading.......................................................................................71
8.3 Running the Gel.......................................................................................71
8.4 Removing the Gel ....................................................................................73
8.5 Staining and Photographing the Gel ........................................................74
Section 9 Troubleshooting Guide ..............................................................75
9.1 Equipment ..............................................................................................75
9.2 Applications .............................................................................................77
Section 10 Specifications..............................................................................81
Section 11 Maintenance ................................................................................82
Section 12 References...................................................................................82
Section 13 Instruments and Reagents for Mutation Detection
Electrophoresis ..........................................................................83