Dcode control and electrophoresis reagents – Bio-Rad DCode™ Universal Mutation Detection System User Manual

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DCode Control and Electrophoresis Reagents

170-9150

DCode Control Reagent Kit for DGGE/CDGE/TTGE, includes primers
(one GC-clamped) and DNA templates for production of wild-type and
mutant DNA

170-9151

DCode Control Reagent Kit for SSCP, includes primers and DNA
templates for production of wild-type and mutant DNA

170-9152

DCode Control Reagent Kit for heteroduplex analysis, includes
homoduplex and heteroduplex DNA in 1x loading buffer

170-9170

DCode Electrophoresis Reagent Kit for DGGE/CDGE, includes 500 ml
40% acrylamide/bis solution(37.5:1), 250 g urea, 225 ml 100% formamide
(deionized), 2 x 1 liter 50x TAE buffer, 10 ml of 10 mg/ml EtBr, 1 ml of 2x gel
loading dye, 10 ml DCode dye solution, 5 ml TEMED, 10 g ammonium
persulfate

170-9171

DCode Electrophoresis Reagent Kit TTGE, includes 500 ml 40%
acrylamide/bis solution(37.5:1), 1 kg urea, 2 x 1 liter 50x TAE buffer, 10 ml
of 10mg/ml EtBr, 1 ml of 2x Gel loading dye, 5 ml TEMED, 10 g
ammonium persulfate

170-9172

DCode Electrophoresis Reagent Kit for SSCP, includes 500 ml 40%
acrylamide solution, 500 ml 2% bis solution, 100 ml of glycerol, 6 x 1 liter
10x TBE buffer, 2x SSCP gel loading dye, 5 ml TEMED, 10 g ammonium
persulfate

170-9173

DCode Electrophoresis Reagent Kit for Heteroduplex Analysis, includes
250 ml 2x DEM solution, 250 g urea, 6 x l liter 10x TBE, 2x gel loading dye,
10 ml of 10 mg/ml EtBr solution, 5 ml TEMED, 10 g ammonium persulfate

170-9174

DCode Electrophoresis Reagent Kit for PTT, includes 500 ml 40%
acrylamide/bis (37.5:1), 2 x 1 liter 10x Tris/Glycine/SDS buffer, 30 ml
Laemmli sample buffer, 500 g Tris base, 25 ml 2-mercaptoethanol, 250 ml
10% SDS, 10 g ammonium persulfate, 5 ml TEMED

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