Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 64)

Fig. 6.5. Aligning spacers in the sandwich assembly.
7. Remove the alignment card. Remove the sandwich assembly from the casting stand and
check that the plates and spacers are flush at the bottom. If the spacers and glass plates
are not flush, realign the sandwich and spacers to obtain a good seal (Repeat steps 5–7).
8. Once a good alignment and seal are obtained, tighten the clamp screws until it is finger-tight.
6.8 Casting SSCP Gels
1. Place the gray sponge onto the front casting slot. The camshafts on the casting stand
should have the handles pointing up and pulled out. Place the sandwich assembly on the
sponge with the shorter plate facing forward. When the sandwich is placed correctly, press
down on the sandwich and turn the handles of the camshaft down so that the cams lock
the sandwich in place.
2. Into a 50 ml tube, add the required amounts of solutions (Section 6.2). Add a final
concentration of 0.09% (v/v) each of ammonium persulfate and TEMED solutions. Cap the
tube and mix.
3. Insert a comb in the gel sandwich and tilt it so the teeth are at a slight angle. This will
prevent air from being trapped under the comb teeth while pouring the gel solution
(Figure 6.6).
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