Bio-Rad DCode™ Universal Mutation Detection System - Manual (Page 63)

Fig. 6.3. Positioning glass plates, spacers, and clamps.
4. Grasp the gel sandwich firmly. Guide the left and right clamps onto the sandwich so that
the long and short plates fit the appropriate notches in the clamp (Figure 6.4). Tighten
the screws enough to hold the plates in place.
Fig. 6.4. Attaching the clamps to the glass plate assembly.
5. Place the sandwich assembly in the alignment slot (the slot without cams) of the casting
stand with the short glass plate forward (Figure 6.5). Loosen the sandwich clamps and
insert an alignment card to keep the spacers parallel to the clamps.
Note: Always use the alignment slot and alignment card to set the spacers in place. Failure
to use these can result in gel leakage when casting, as well as buffer leakage during the
run.
6. Align the plates and spacers by simultaneously pushing inward on both clamps at the
locating arrows while, at the same time, pushing down on the spacers with your thumbs.
Tighten both clamps just enough to hold the sandwich in place. Pushing inward on both
clamps at the locating arrows will insure that the spacers and glass plates are flush against
the sides of the clamps (Figure 6.5).
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