Bio-Rad DCode™ Universal Mutation Detection System User Manual
Page 68
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Coomassie Blue Destain
Reagent
Amount
Final Concentration
Methanol
400 ml
40%
Acetic acid, glacial
100 ml
10%
dH
2
O
500 ml
Total volume
1,000 ml
Mix and store at room temperature.
7.3 Gel Volumes
The table below provides the required volume for the gel size and spacer thickness.
Spacer Thickness
20 x 20 cm Gel
0.75 mm
30 ml
1.00 mm
40 ml
7.4 Sample Preparation
1. Dilute samples, controls, and protein marker (such as Bio-Rad pre-stained markers) 1:2
with Laemmli sample buffer.
Note: Add 10 µl
b-mercaptoethanol to 590 µl Laemmli buffer just before use. This solution
is good for 1 day only.
2. Heat the samples at 95–100°C for 5 minutes.
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