Bio-Rad Image Lab™ Software - Manual (Page 122)

5 | Viewing Images
122 | ChemiDoc MP Imaging System with Image Lab Software
The zoom tools work the same way they work elsewhere in the software. For more
information, see Zoom Tools on page 103 .
In addition to the graph of the lane intensities, the Lane Profile tool also shows an
image of the selected lane below the graph. The multichannel channel of
multichannel images is always a gray scale image of the lane with the default
transform applied to that lane. For single-channel images and individual channels of
a multichannel image, the transform and color map are applied to the gray-scale
image.
As you move your cursor over the profile, the current relative front (Rf) value and the
average intensity (Int) value at the Rf value display in the lower-right corner of the
Lane Profile window.
Scale to Fit Graph
You can choose the highest point of the display to define the range of the graph.
This provides the best view of the lane profile.
You can clear the Scale to Fit Graph checkbox to display the entire range of
possible intensity values in the graph. Doing so allows valid comparisons between
different lanes.
Include Background
When the Include Background checkbox is selected, the Lane Profile window
shows the subtracted background under the blue line. The area used for band
quantification appears in green under the red line.
When you clear the Include Background checkbox, the area of the lane profile that
represents the background of the image does not display.