Bio-Rad Image Lab™ Software - Manual (Page 76)

4 | Acquiring Images
76 | ChemiDoc MP Imaging System with Image Lab Software
For a list of applications with all required detection reagents, light sources, and
any conversion screens or filters noted, see Application Tables on page 97 .
3. In Image Exposure, if you select the first radio button, Image Lab acquires an
image estimating an optimal exposure time. This option ensures the best use of
the dynamic range.
 If you choose Intense Bands from the list, exposure is optimized for all
bands.
 If you choose Faint Bands from the list, a longer exposure makes faint
bands more visible, but more prominent bands might be overexposed.
 If you select the second radio button to manually override automated
imaging, you can set the exposure time from 0.001–7,200 seconds.
After imaging a gel with automatic exposure optimization, the exposure time
displays in the protocol so you can manually adjust it if needed.
Note: You can also view the exposure time of the image later, in the Image
Info window (see Image Info on page 109 ).
Signal Accumulation Mode — if you are running a chemiluminescence
application on the ChemiDoc MP imager, you can also use signal accumulation
mode (SAM).