Bio-Rad Image Lab™ Software - Manual (Page 149)

Optimizing Normalization of Volume Data
User Guide | 149
Subtract any Extraneous Background
The lane volume data is background-subtracted. Review the background
subtraction being applied to each lane by using the Lane Profile tool. Verify that an
equivalent background profile is being subtracted from each lane in the
normalization channel so the normalization factor is accurate. For more information
on the lane Background Subtraction tool, see Volume Background Subtraction on
page 172 .
Remove Compromised Data
There are several reasons why lanes should be excluded from your analysis:
 Empty lanes — the first nonstandard lane in the selected channel is used
as the normalization factor against which all other lanes in all the channels
are compared. If the first nonstandard lane is not a valid lane (for example,
an empty lane), delete it from the channel.