Bio-Rad Image Lab™ Software - Manual (Page 67)

User Guide | 67
4 Acquiring Images
Image Lab
 software runs specific applications with repeatable workflows using
configurable protocols that have a wide variety of settings. These protocols can be
retrieved, revised, and reused.
In Image Lab, a protocol is any combination of settings for imaging, analyzing, and
reporting that runs as a single workflow.
Image Lab supports two kinds of protocols: single-channel and multichannel. A
single-channel protocol enables you to choose one application for acquisition of a
single image from a gel or blot, with the exception of signal accumulation mode for
chemiluminescence. Multichannel protocols allow you to sequentially acquire up to
three separate images of a gel or blot, using different illumination, filter, and
exposure settings, to create a combined image. The multichannel image can be
viewed as an RGB (red, green, blue) color-composite image.
Note: The Image Lab software is designed to work with different imagers,
some of which can only produce single-channel images. The New Protocol
menu choices change depending on whether the imager supports multichannel
images or not. If you are using a ChemiDoc™ MP imager and you do not see
the multichannel option, check to see that your default imager selection is set
to ChemiDoc MP and not one of the single-channel imagers.
To create a new protocol, do one of the following:
 Click the New Protocol button on the toolbar, and then select Single
Channel or Multichannel in the menu that appears.
 In the Protocols box on the Start Page, click New Single Channel or New
Multichannel.
The appropriate Protocol Setup window appears for the type of protocol you
selected.