Bio-Rad Image Lab™ Software - Manual (Page 146)

6 | Analyzing Images
146 | ChemiDoc MP Imaging System with Image Lab Software
Detect the Lanes in the Channels
Start by detecting the lanes in your multichannel image. You can use either the
automatic or the manual lane detection method. For more information on when to
use each method, see Lane and Bands Tools on page 133 . If your multichannel
image consists of unlinked images, you will need to pay particular attention to the
lanes that are detected to ensure that the corresponding lanes are correctly mapped
for each channel. For example, you can use the Copy Lanes tool to map your lanes.
For more information on that tool, see Copying Lanes on page 138 .
Make Adjustments to the Lanes
Regardless of which method you use to detect your lanes, you should review the
lane borders to ensure that each lane encompasses all the protein in a particular
lane, and that the lane does not overlap into another lane. For example, in the
following figure, lanes were detected using the automatic lane finder. Some of the
lanes exclude material that should be included.
Note: Image Lab automatically excludes standard lanes from normalization.