Bio-Rad Image Lab™ Software - Manual (Page 77)

Creating a Protocol
User Guide | 77
SAM is used to simplify the capture of a good image from a chemiluminescent
sample. This sample type often requires long integration times to obtain an
image that represents the best range of signal.
Rather than manually acquiring a series of independent images with different
imaging times, SAM presents a series of cumulative images with progressively
greater signal in each image. To run SAM you must estimate the shortest and
longest times expected to generate an image with the appropriate signal
intensity. You then decide how many total images to acquire in this window of
time.
For example, if the minimum expected time to image the sample is 1 minute
and the maximum is 5 minutes, these values are entered (in sec) in the setup
window. By entering 5 in the Total number of images field, three images will be
acquired between the first and last images.
To use SAM, click Signal Accumulation Mode and select Setup to display the
Signal Accumulation Setup dialog box.
In this example, the bar in the Signal Accumulation Setup dialog box indicates
that images will be acquired at 1 minute intervals, starting at 1 minute and
ending at 5 minutes. The second 1 minute image is added to the first 1 minute
image, and the final image is the result of integrating these two images. The
third 1 minute image is added to the previous image, and so on, until the last
image is presented.