Bio-Rad Image Lab™ Software User Manual
Page 153
Optimizing Normalization of Volume Data
User Guide | 153
3.
To add a second image, double-click on an image from the Compatible Open
Images list.
If no images appear in the list or you want to use a different image, click Browse
to navigate and select the desired image.
Note: Image Lab checks to see if the image has the same aspect ratio as
the Sample Data image. If the aspect ratio is not compatible with the
Sample Data image, it will not appear in the list of Compatible Open
Images. The aspect ratios might not be compatible if they were created
using different instrument models (for example, Gel Doc™ EZ and
ChemiDoc™ MP) or if the images are cropped at different aspect ratios.
Tip: You can move an image to the Normalization Data box by dragging it
from the Compatible Open Images list, and you can remove it by dragging
it back to the list.
4.
Click OK.
The Auto-Analysis window appears, prompting you to detect the lanes and
bands in your images.
5.
In the Auto-Analysis window, select the level of sensitivity and click OK.
6.
Follow the Normalization Steps listed in the pane to get the best results.
Optimizing Normalization of Volume Data on page 145
for an explanation
of each step in the process.