Bio-Rad Image Lab™ Software - Manual (Page 78)

4 | Acquiring Images
78 | ChemiDoc MP Imaging System with Image Lab Software
Although SAM is useful for determining the optimum imaging time for a
chemiluminescent sample, it results in data that are not as accurate as data
from a single image. Signal that is near the intensity of background noise
becomes increasingly masked as the number of cumulative images grows. To
identify extremely faint signals in an image, reacquire it as a single image, using
the time the SAM tool found to be appropriate.
4. Set the following attributes in Display Options:
 Highlight saturated pixels — select this checkbox to see any saturated
pixels in red. This shows how much of the gel image is saturated. You can
change this option later by selecting View > Image Transform.
 Channel Color — select a color to display the sample image. Assigning
each channel a different color makes it easy to identify channels. After you
set up the first channel, the second channel box becomes active.
5. Repeat steps 1 – 4 to set up the second and third channels, if applicable. The
software determines which channels are available based on the previously
selected applications. Each Configure box displays the settings for that
channel.
You can reconfigure channel settings by clicking Configure in the channel box
and changing the settings.