Bio-Rad Image Lab™ Software - Manual (Page 187)

User Guide | 187
9 System Calibration
When your imager is installed, instrument calibration is performed with a calibration
wizard. See the installation guide in your Image Lab™ upgrade kit for detailed
instructions.
The instrument calibration wizard performs several procedures required to automate
the system and prevent focus problems. Each of these calibrations affects your
system as follows:
 Focus Calibration — this calibration allows automated focus settings at
any zoom point, using a (patent pending) software algorithm. Therefore,
your focus remains correct whether you view an entire sample or an area of
interest.
 Focus Calibration with Height Offset — this calibration takes the tallest
of the available conversion screens into account, and extrapolates values
for the others, so that focus remains optimal, for whichever screen is used.
 Dark Reference Image — this calibration determines and corrects any
background signal present in your imager
 UV Flat Field Calibration — this calibration generates the flat field correction
profiles needed for the UV light source. Because of this calibration, your
images have more accurate quantity reporting and backgrounds of even
intensity.
 Lens Flat Field Calibration — this calibration corrects for the intensity roll-
off inherent in any lens.
 White Conversion Screen Calibration — this calibration generates a flat
field correction profile needed for the white light conversion screen.