Bio-Rad Image Lab™ Software - Manual (Page 158)

6 | Analyzing Images
158 | ChemiDoc MP Imaging System with Image Lab Software
The values for the molecular weight in any standard lane appear in bold. In this
example, the values for the first lane are in bold. (Lanes 16 and 17 are not included
in the screen shot, but their values would also be in bold.) The molecular weight of
the bands in the remaining lanes is calculated relative to these standards.
Molecular Weight Analysis in Multichannel Images
Molecular weight analysis is performed differently in multichannel images
depending on whether the channels of the image are linked or unlinked. If the
channels are linked, then the standard lanes are synchronized across all channels. If
the channels are unlinked, each channel has its own standard.
When you select the standard lane in one channel of a linked multichannel image,
the same lane is selected as the standard in all other channels. The combined data
in the multichannel image are used to detect the bands in this standard lane and
these bands are synchronized across all channels. All the bands in all the channels
are calculated using the same standard lane. If you deselect this lane as the
standard, the bands in the standard lane are deleted from all channels, including the
multichannel image.
With unlinked multichannel images, each channel is treated independently as if it
were a single-channel image. You select a standard lane for each channel and the
molecular weight is calculated for only the bands in that channel.
In the following screen shot, lane 1 in the linked multichannel image is selected as
the standard lane for all channels. Because the images are linked, the same
molecular weights for the standard lane and the same red lines showing the location
of the bands in the standard lane are displayed in all channels.