Bio-Rad Bio-Plex System Validation and Calibration Tools User Manual
Page 14
7.
Place 5 drops each of bead into the respective wells on the MCV plate II
(see Figure 3 below or Figure 2B). Store beads at 4°C as soon as possible
after use. Protect the beads from light.
Fig. 3. MCV plate II.
8.
Select the Eject button in the dialog box to eject the plate holder.
9.
Place the MCV plate II in the microplate platform.
10. Select OK to begin all validation procedures.
9.2 Validation of Optics Alignment
Procedure
1.
Turn on the Bio-Plex array reader, microplate platform, and computer as
specified in the Bio-Plex hardware and Bio-Plex Manager user manuals.
2.
Perform start-up procedure as directed.
3.
Calibrate Bio-Plex array reader using CAL1 and CAL2 beads found in the
Bio-Plex Calibration Kit according to the Bio-Plex Manager software manual.
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4.
Remove the optics validation bead set from 4°C storage and vortex each
bottle for 30 sec.
5.
Place 5 drops each of optics beads 1 and 2 into the respective wells on the
MCV plate II.
6.
Store optics beads at 4°C as soon as possible after use. Protect the beads
from light.
7.
Select Instrument from the main menu. Select Validation from the pull-down
menu.
Bio-Plex Manager 3.0 and MCV Plate II Instructions
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