Bio-Rad Bio-Plex System Validation and Calibration Tools User Manual

Page 27

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3.

Place 5 drops of each reporter bead into the corresponding reporter well
labeled as B (blank), 1, 2, 3, 4, and 5 in the MCV plate (see Figure 2).

4.

Store reporter beads at 4°C as soon as possible after use. Protect the beads
from light.

5.

Fill the DI H

2

O reservoir with water.

6.

Select Instrument from the main menu. Select Validation from the pull-down
menu. A dialog box will appear. Select Reporter from the pop-up menu. The
following dialog box appears:

Fig. 15. Reporter validation dialog.

7.

Select the Eject icon in the dialog box to eject the plate holder.

8.

Place the MCV plate in the Bio-Plex microplate platform.

9.

Select OK to start the reporter validation procedure.

10. When the procedure is completed, values will be displayed in a dialog box as

shown below:

Fig. 16. Reporter validation results.

Bio-Plex Manager 2.0 and MCV Plate Instructions

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