Bio-Rad Bio-Plex System Validation and Calibration Tools User Manual
Page 18
9.4 Validation of Reporter Channel Performance
Procedure
1.
If not already done, follow the procedure for start-up and calibration of the
Bio-Plex system.
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2.
Remove the reporter validation bead set from 4°C storage and vortex each
bottle for 30 sec.
3.
Place 5 drops of each reporter bead into the corresponding reporter well
labeled as B (blank), 1, 2, 3, 4, and 5 in the MCV plate II (see Figure 2).
4.
Store reporter beads at 4°C as soon as possible after use. Protect the beads
from light.
5.
Fill the DI H
2
O reservoir with water.
6.
Select Instrument from the main menu. Select Validation from the pull-down
menu. A dialog will appear. Enter user name and control number. Select
Reporter Validation. Select OK. The following dialog appears: (Figure 5)
Fig. 8. Reporter validation dialog.
7.
Select the Eject icon in the dialog box to eject the plate holder.
8.
Place the MCV plate II in the microplate platform.
9.
Select OK to start the reporter validation procedure.
10. When the procedure is completed, values will be displayed in a dialog box as
shown below: (Figure 7)
Bio-Plex Manager 3.0 and MCV Plates II Instructions
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