Bio-Rad Bio-Plex System Validation and Calibration Tools User Manual
Page 24
Section 10
Procedure for Bio-Plex Manager 2.0
and MCV Plate
10.1 Validation of Optics Alignment
Procedure
1.
Turn on the Bio-Plex array reader, microplate platform, and computer as
specified in the Bio-Plex hardware and Bio-Plex Manager user manuals.
Note: Be sure to calibrate immediately before validation. Use the High RP1
Target value for CAL2 calibration.
2.
Perform start-up procedure as directed.
3.
Calibrate Bio-Plex array reader using CAL1 and CAL2 beads found in the
Bio-Plex calibration kit according to the Bio-Plex Manager software manual.
4.
Remove the optics validation bead set from 4°C storage and vortex each
bottle for 30 sec.
5.
Place 5 drops of optics bead 1 into the first of the 3 optics wells labeled with
"1" on the MCV plate (see Figure 2 below). Leave the 2 remaining wells
labeled with "1" empty.
Fig. 12. MCV plate.
6.
Place 5 drops of optics bead 2 into the first of the 3 optics wells labeled with
2 on the MCV plate. Leave the 2 remaining wells labeled with "2" empty.
7.
Store optics beads at 4°C as soon as possible after use. Protect the beads
from light.
Bio-Plex Manager 2.0 and MCV Plate Instructions
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