Prime the reagent packs – Dako Artisan™ Link Pro User Manual

Page 46

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Dako Artisan™ Link

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Basic User Guide

Note:

The slides should soak in wash solution for 5-10 minutes prior to loading

onto Artisan™ Link.

1. One at a time, remove slides from wash solution.
2. Carefully dry moisture from the back of the slide with a paper towel or absorbent gauze. Be

sure to wipe the back of the frosted end of the slide as well.

3. Place the slide with the tissue side up and the slide label end towards you within the guide

rails of the slide position.

4. Place a slide clip over a slide with the hook end facing upward and secured into the loop at

the back of the slide position.

5. Press down on the front end of the clip until it clicks into place and slowly push the clip

towards the back of the slide to ensure a proper seal.

6. Using a squeeze bottle, add 1X wash solution to the slides until the entire slide is covered

with a layer of liquid (approximately 3 mL). Be careful to not drip the wash solution between

the slides.

Note:

Before staining begins, ensure that tissue sections never become dry.

Drying the tissue sections will cause drying artifacts.

7. Repeat this process until all slides are loaded onto the Artisan™ Link.

Prime the Reagent Packs

You must prime the reagent packs when you:

Use a new staining reagent

Start a new reagent pack

Start your first run of the day

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