Luminex xPONENT for MAGPIX - Manual (Page 123)

CL2 Refers to dyes embedded in the microsphere. Also see classification
channel.
classification channel A specific range of wavelengths in which light intensity is measured.
Includes the fluorescence emission of a given classification dye.
Classification channels are abbreviated as CL1 and CL2.
CSV Comma separated value file format used to output data from the
application.
data reduction The analysis of acquired batch data.
delta cal temperature The difference between the current temperature of the Doublet
Discriminator APD and its temperature at your last calibration. The
system displays this value on the Diagnostics tab within the software.
Once the delta cal temperature exceeds +/- 3ºC, the system must be
recalibrated.
dot plot Graphical representation of microsphere analysis classification data
displayed by frequency and intensity.
emission spectrum Wavelength range that an excited fluorochrome emits when its electrons
fall from a higher to a lower energy state. Expressed in nanometers (nm).
excitation spectrum Wavelength range that excites a molecule’s electrons to a higher energy
state. Expressed in nanometers (nm).
fluorescence Light emission that occurs when the electrons of a fluorochrome drop to
a lower energy state.
fluorochrome A fluorescent molecule.
fluorophore See fluorochrome.
Immunofluorescence A technique which uses a covalently linked fluorochrome-antibody
complex to detect or quantify a particular antigen.
kit A group of standards and controls used to create a protocol. They are
used in the protocol’s definition for the multi-analyte assays that will be
performed. Formerly called “product” in previous releases of Luminex
software.
Luminex xMAP
microsphere set Luminex multi-analyte microspheres containing a unique mixture of two
distinctly colored fluorochromes to distinguish them from other multi-
analyte microspheres.
LIS laboratory information system; a system for storing sample records and
test requests.
lot A reagent with specific target values used for analysis or calibration.
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