Metrohm 889 IC Sample Center - Manual (Page 31)

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4 Functioning
889 IC Sample Center
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￭ 2 × loop volume with sample loops 100...500 µL
￭ 1.5 × loop volume with sample loops ≥ 500 µL
5 Switching the injection valve to INJECT
The injection valve switches to the INJECT position. The sample loop
is now part of the flow path of the eluent; the sample is transported
to the separation column. The determination is started.
A washing cycle is performed after each injection.
Air segment with the full loop injection
A 5 µL air segment can be used in order to reduce the necessary volume
of sample solution. The air segment must be located at the head of the
rinsing volume and will not be injected.
In the case of a standard needle, the rinsing volume with air segment
must be at least 30 µL for one injection, and at least 35 µL for injections
without air segments. In the case of high-viscosity samples, larger rinsing
volumes must be programmed and the speed of the syringe stroke must
be reduced in order to improve reproducibility.