Metrohm 850 Professional IC Anion MSM-HC - LP-Gradient - Manual (Page 104)

5.13 Separation column
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850 Professional IC Anion MSM-HC LP Gradient
3 Mounting filter screw
￭ Screw the filter screw (45-3) back into the tubing olive (45-1) and
tighten by hand. Then additionally tighten with two 6.2621.000
adjustable wrenches.
5.13 Separation column
5.13.1 Separating efficiency
Which analysis quality can be attained, depends to a great extent on the
separating efficiency of the separation column used. The separating effi-
ciency of the selected separation column must be sufficient for the analy-
sis problems present. If difficulties occur, you should always first check the
quality of the separation column by recording a standard chromatogram.
You can find detailed information on the separation columns available
from Metrohm in the information sheet provided along with your separa-
tion column, in the Metrohm IC-Column Program (available via your
Metrohm agent) or in the Internet at
http://www.metrohm.com in the
product area Ion chromatography. You can request free information on
special IC applications in the corresponding "Application Bulletins" or
"Application Notes", which are available in the Internet at
http://
www.metrohm.com in the Applications area or via the Metrohm agent
responsible.
5.13.2 Protection
To protect the separation column against foreign particles, which can
affect the separating efficiency, we recommend that both the eluent and
the samples undergo a microfiltration (filter 0.45 µm) before being aspira-
ted via the 6.2821.090 aspiration filter.
The use of guard columns (see Chapter 3.21, page 61) serves for protect-
ing the actual separation columns and increases their service life consider-
ably. Information regarding which guard column is suitable for your sepa-
ration column can be seen in the Metrohm IC Column Program (which
is available from your Metrohm agent), the information sheet provided
along with your separation column, the product information on the sepa-
ration column at
http://www.metrohm.com (product area Ion Chroma-
tography) or can be obtained directly from your agent.
The pulsation damper (see Chapter 3.14, page 44) must be installed in
order to protect the column material from pressure concussion caused by
injection.