Metrohm 850 Professional IC Anion MSM-HC - LP-Gradient - Manual (Page 110)

6.1 Problems and their solutions
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100 ￭￭￭￭￭￭￭￭
850 Professional IC Anion MSM-HC LP Gradient
Problem Cause Remedy
Precision problems -
significant scatter-
ing of the measured
values Sample – Gas bubbles in
the sample. Use sample degasser (see Chapter 3.15, page
45).
Injection valve – sample
loop. Check installation of the sample loop (see
Chapter 3.16.1, page 47).
Sample – Rinsing volume
too low. Prolong the rinsing time (see Chapter 5.8,
page 83).
Injection valve – defective. Request Metrohm Service.
Unexpected change
to the retention
times in the chroma-
tograms Separation column –
diminished separating effi-
ciency. ￭ Regenerate separation column (see Chap-
ter 5.13.4, page 95).
￭ Replace separation column (see "Connect-
ing and rinsing the separation column",
page 63).
Eluent – Gas bubbles in the
eluent. Check connections of the eluent degasser (see
Chapter 3.10, page 33).
High pressure pump –
defective. Request Metrohm Service.
Vacuum is not being
built Eluent Degasser – Connec-
tor Vacuum on the rear of
the instrument not (tightly)
sealed. ￭ Seal the connector Vacuum tightly with a
6.144.6040 threaded stopper.
or when using an 872 Extension Module:
￭ Connect an FEP tubing between the IC
instrument and the Extension Module and
tighten both clamping screws until they
seal.