Metrohm 790 Personal IC - Manual (Page 62)

3.3 Calibration
790 Personal IC
53
3.3 Calibration
After the complete IC system has been installed as described in sec-
tion 3.2, the first calibration can be started. A standard solution is re-
quired for this; it should contain the substances to be determined in
approximately the same concentrations in which they can be expected
in the sample.
In our example of drinking water determination using the METROSEP
Cation 1-2 IC cation column a 10 µL sample loop is used; this is filled
with the following standard solution:
1
mg/L Li +
5 mg/L Na + and NH
4
+
10 mg/L K + , Ca 2+ and Mg 2+
Please note that all program displays refer to the condition in which the
system Cation.smt is loaded for the first time. If you want to work through
this tutorial at a later date and the system Cation.smt has been altered in
the meantime then differences with respect to the program display and
the parameter values may occur.
In the description of the calibration procedure, it is assumed that the PC
and 790 Personal IC are not in operation and that the system must first
be conditioned again. If this is not the case (e.g. if you start the tutorial
immediately after conditioning) then you can skip steps 1 to 5.
1 Switch on 790 Personal IC
⇒ Switch on 790 Personal IC with mains switch 11 11 11 11 on the rear of
the instrument. After the instrument has been switched on the
mains pilot lamp 2 222 lights up.
2 Switch on PC
⇒ Switch on PC and start «790 Personal IC» program.
3 Open system " Cation.smt "
⇒ Select File / Open / System in the main window. Select the
system file Cation.smt in the folder 790com1 or 790com2 and
click on <Open> .
4 Start system " Cation.smt "
⇒ Select Start determination of the Control menu in the system
window. The high-pressure pump is started, at the same
time, a chromatogram window opens where the baseline is
recorded continuously.
5 Condition system " Cation.smt "
⇒ Rinse IC system with eluent until the desired stability of the
baseline is achieved (at least 1 h).