Volume measurement definitions, Ement type, see, Volume measurement – Bio-Rad Image Lab™ Software User Manual

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Band % — percentage of the band’s volume compared to all band volumes in the

lane.

Lane % — percentage of the band’s volume compared to the entire volume of the

lane.

Volume Measurement Definitions

This section defines the measurements that display in the Volume tab in the Analysis

table. Use the Display Data Options button to choose the columns to display.

Volume Number — a unique number is assigned to each volume.

Volume Label — software-generated labels for different types of volumes

(U

unknown, B – background, S – standard). Label can be changed in Volume

Properties.

Volume — the sum of all the intensities within the band boundaries.

Adjusted Volume — the background-adjusted volume.

Mean Background — the mean value of the background.

Absolute Quantity Volume — the quantity of the volume based on the standard

volumes and the regression method.

Relative Quantity Volume — the ratio of the adjusted volume and the adjusted

volume of the reference volume.

# Pixels — number of pixels inside the volume boundary.

Minimum Value — intensity of the pixel with the minimum intensity inside the

volume.

Maximum Value — intensity of the pixel with the maximum intensity inside the

volume.

Mean Value — mean value of all pixels inside the volume boundary.

Standard Deviation — standard deviation of all pixels inside the volume boundary.

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