Bio-Rad Image Lab™ Software User Manual
Page 81
Creating a Protocol
User Guide
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a.
Enter a name for the new standard.
b.
Choose a standard type: protein or nucleic acid
A protein standard can be expressed in either isoelectric point (pI) or
molecular weight (kDa) units. A nucleic acid standard can be expressed in
base pairs (bp), kilo base pairs (kb), or mega base pairs (Mb) units.
c.
Click Add to display the Edit dialog box.
Note:
At least two values must be added to create a new standard.
d.
Enter the value and description of your new standard and click OK to
return to the Edit Standard dialog box.
e.
Click Add to display the Edit dialog box again, and enter a second value
and description for your new standard.
f.
Click OK to save your changes and close the Edit dialog box.
g.
Click OK in the Edit Standard dialog box to save your new standard.
h.
Click OK in the Manage Standards dialog box to save your changes.
4.
Choose the lane(s) that contain your standards by typing lane numbers or the
words First and Last in the Standard Lanes field. The format is xx, xx, xx, and
so on, where xx is the lane number. For example, if you run an 18-well gel and
want your standards in lanes 1, 10, and 18, enter First, 10, Last.
Note:
Lane detection works best when standards are placed in the first
and last lanes. For nucleic acid samples, use this step to determine the
size of the bands in base pairs.
For more information, see
Molecular Weight (MW) Analysis Tools on page
5.
Select the appropriate regression method for the gel type: