Bio-Rad Image Lab™ Software - Manual (Page 226)

E | Using Bio-Rad’s Stain-Free Technology
226 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software
Imaging Blots
To blot stain-free gels, use standard blotting procedures as described in the
instruction manual you are using. Use only PVDF membranes with low background
fluorescence, as membranes other than low fluorescence PVDF can result in high
background or low sensitivity with the ChemiDoc XRS+ and Gel Doc XR+ imagers.
To assess transfer efficiency, be sure to activate and visualize the gel using the
imager before transfer.