Orange fluorescence reference plate – Bio-Rad Image Lab™ Software User Manual

Page 216

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216 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

4.

Place the white light transilluminator on top of the UV transilluminator.

Your system must be calibrated to use the white light transilluminator.

To calibrate the system to use the white light transilluminator

Choose Edit > Instrument Setup and select the appropriate checkbox in the
dialog box. The Image Lab™ software will guide you through the calibration.

Orange Fluorescence Reference Plate

The orange fluorescence reference plate enables you to apply UV flat fielding

corrections to your imager. Corrections are made for all UV illumination sources,

filters, and the camera lens.

The orange fluorescence reference plate is recommended for quantitation with

Bio-Rad’s stain-free gels, and can be used to correct for image nonuniformities for

red and orange gels, including:

Ethidium bromide

GelRed

Flamingo™

Coomassie Fluor Orange

SYPRO Ruby

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