Bio-Rad Image Lab™ Software - Manual (Page 77)

Creating a Protocol
User Guide | 77
Although SAM is useful for determining the optimum imaging time for a
chemiluminescent sample, it results in data that are not as accurate as
data from a single image. Signal that is near the intensity of background
noise becomes increasingly masked as the number of cumulative images
increases. To identify extremely faint signals in an image, reacquire it as a
single image, using the time the SAM tool found to be appropriate.
4. In Display Options, you can set:
 Highlight saturated pixels — select this checkbox to see any saturated
pixels in red. This shows how much of the gel image is saturated. This
option can be changed later by selecting View > Image Transform.
 Image color — select color choice to display the sample image. Viewing
the image with a different color scheme can make it easier to see all of the
elements. See Image Colors on page 102 to view the color choices dialog
box.