Running a protocol – Bio-Rad Image Lab™ Software User Manual

Page 88

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6

| Acquiring Images

88 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

Running a Protocol

To run a protocol

Click Run Protocol in the left pane.

The software runs through the steps in imaging mode and a progress indicator

tracks the progress.

Note:

If you run a chemiluminescence application using autoexposure, you

might see the following error message:

The sample is too faint to use the autoexposure setting. Change the Image

Exposure setting to use manual exposure or Signal Accumulation Mode (SAM), then

run the protocol again.

This error occurs if Image Lab, using the initial autoexposure algorithm, determines

that it would take too long (approximately 30 minutes or more) to acquire an image.

You can always use the manual Image Exposure setting and specify a longer

exposure time.

After the images are taken, Image Lab continues with the detection, analysis, and

reporting steps if these steps were selected in the Protocol Setup pane. When

Image Lab has completed running the protocol, it displays the images that were

acquired. You can then edit and save these images or do further analysis on them.

To end the protocol

Click Cancel Run.

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