Dietary fiber analysis support items, Analysis options using the ankom, Dietary fiber analyzer – ANKOM TDF Dietary User Manual

Page 16: Blanks, Operator’s manual

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Operator’s Manual


pg. 16

Rev E 12/2/14

Dietary Fiber Analysis Support Items

The following support items needed to perform the fiber analysis may be purchased separately:

Item

ANKOM Part #

Electronic Balance with four-place readout

TB

Electronic Balance Software

TBS

Heat Sealer for sealing the filter bags

1915 (120V), 1920 (220V)

IDF Filter Bags

DF-I

SDF Filter Bags (used for SDF and TDF procedures)

DF-S

IDF Flow-Thru Bags (TDF procedure only)

DF-FT

Solvent Resistant Marker

F08

Rinse Stand

TDF51

Diatomaceous Earth (DE)

XTC

Drying Oven

Ashing Oven

Protein Determination Equipment (Kjeldahl is recommended)

Analysis Options using the ANKOM

TDF

Dietary Fiber Analyzer

The ANKOM

TDF

Dietary Fiber Analyzer can be configured to recover IDF and SDF residue, or to recover TDF residue. The

following describes these analyses along with their specific filter bag configurations.

Analysis Name

Analysis Description

Upper Bag

Lower Bag

IDF/SDF

(991.43)

This analysis is used to determine IDF and SDF residue separately
using two different filter bags. TDF is calculated by adding the IDF
and SDF residue weights corrected for protein and ash content.

IDF

SDF

TDF

(991.43 & 985.29)

This analysis is used to determine TDF residue in one filter. In this
analysis, the TDF value consists of the weighed residue corrected
for protein and ash.

IDF Flow-Thru

(no filter)

SDF

A computer controlled Diagnostics mode is provided through the Control Panel for maintenance and troubleshooting purposes.

To maximize productivity, this instrument also allows a user to start new procedures (IDF/SDF and TDF) before the previous
ones have completed. See the “Productivity Enhancement” section of this manual for details.

Contact ANKOM about an upgrade to Total Integrated Dietary Fiber (AOAC
2009.01 and 2011.25 methods). See the Diagnostics section of this document for
instructions on how to enable these methods.

Blanks

BLANK values are used in the IDF, SDF, and TDF calculations. The following BLANK values (based on extensive research
done in the ANKOM lab) are the default values loaded in the IDF_SDF and TDF spreadsheets in the MS-Excel workbook
provided with the instrument.

IDF (AOAC 991.43 method): -0.0072

TDF (AOAC 991.43 method): -0.0047

SDF (AOAC 991.43 method): -0.0030

TDF (AOAC 985.29 method): -0.0028

You do not need to run BLANKS with every set of samples. However, if you want to determine your own BLANK values,
especially when you change chemical lots, ANKOM recommends that the BLANK value in the IDF, SDF, and TDF
calculations be the average of at least 18 BLANK values.

If you determine your own BLANK values, you can overwrite the values currently in the MS-Excel spreadsheets.

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