ANKOM TDF Dietary - Manual (Page 93)

Operator’s Manual
Rev E 12/2/14
pg. 93
5. Soak the bags in Petroleum Ether.
5.1 Place all XT4 Filter Bags into a beaker or other container.
5.2 Pour enough Petroleum Ether in the container to cover the bags and allow them to
soak in the solvent for 10 minutes.
6. Remove the Filter Bags from the container and allow the solvent to
evaporate until the bags are completely dry.
7. Carefully cut the XT4 Filter Bags inside the IDF Filter Bags or the IDF
Flow-Thru Bags on the TDF instrument.
To get the XT4 Filter Bag pieces into the TDF bags, hold each XT4 Filter Bag inside
the appropriate TDF bag and cut the XT4 into approximately a dozen pieces being
careful not to lose any sample. If any sample sticks to the scissors, brush it off into the
TDF bag.
8. For a TDF analysis, make sure all XT4 pieces drop from the IDF Flow-
Thru Bags into the SDF Filter Bags.
Use tongs if necessary to help move the pieces.
9. When performing the Ashing procedure, use an XT4 ash BLANK value in the calculation.
10. When performing the Kjeldahl procedure, in order to appropriately digest the extra XT4 bag
material, add 4-5ml more sulfuric acid than is specified in the "Protein Determination" section of
this Operator's Manual.
The XT4 Filter Bags do NOT contain any protein.
A small amount of PTFE polymer will NOT digest during the Kjeldahl procedure.
This is of NO concern.