ANKOM TDF Dietary - Manual (Page 29)

Operator’s Manual
Rev E 12/2/14
pg. 29
22.6 With your Heat Sealer set between 3 and 4 (settings may vary depending on
the heat sealer and the power source), press the Heat Sealer arm down for 3
to 4 seconds to seal each bag just above the filter. This keeps all residue
contained to the filter area while handling the bags.
22.7 Place each bag in the Drying Rack.
23. Rinse the SDF filter bags with acetone.
After the instrument has completed an SDF process the SDF residue that has been collected in the SDF bag must be
manually rinsed with acetone. It is recommended that you use an ANKOM TDF51 Rinse Stand for rinsing filter bags with
acetone.
To rinse the SDF filter bags with acetone using the ANKOM TDF51 Rinse Stand, follow the steps below.
23.1 Remove the SDF bags from the instrument.
23.2 Place the bags on the Rinse Stand by sliding the back part of each bag under the pinch mechanism. Keep the top
of the bag open.
23.3 Using a wash bottle, squirt acetone into each bag, making sure that all residue on the clear polypropylene surfaces
is rinsed down into the filter.
23.4 Repeat step 23.3 so that each bag gets rinsed a total of two times.
23.5 Allow acetone to evaporate from the bags.
Hazardous Materials – Do NOT heat seal or place bags in an oven until all
acetone has evaporated.
23.6 Seal each bag just above the filter to keep all residue contained to the filter area while handling the bags.
Sealing each bag as close to the filter as possible allows you to trim the maximum
amount of polypropylene from the bag before determining the protein content.
23.7 Place each bag in the Drying Rack.
24. Dry the IDF and SDF filter bags.
24.1 Make sure your oven is 105°C at the location where the bags will be placed.
24.2 Place the Drying Rack with the filter bags in the oven and dry to constant weight (about 90 minutes).
24.3 When dry, remove all IDF and SDF filter bags from the oven and place them in separate desiccant pouches.
25. Weigh the IDF residue.
25.1 Remove only one IDF filter bag from the desiccant pouch at a time.
25.2 Roll or fold the bag, place it in a tared Bag Weigh Holder (BWH), and place the BWH in the center of a balance.
25.3 Record the weight of the filter bag.
25.4 Repeat steps 25.1 – 25.3 for each IDF filter bag used in the fiber analysis.
26. Weigh the SDF residue.
26.1 Remove only one SDF filter bag from the desiccant pouch at a time.
26.2 Roll or fold the bag, place it in a tared Bag Weigh Holder (BWH), and place the BWH in the center of a balance.
26.3 Record the weight of the filter bag.
26.4 Repeat steps 26.1 – 26.3 for each SDF filter bag used in the fiber analysis.