ANKOM TDF Dietary - Manual (Page 45)

Operator’s Manual
Rev E 12/2/14
pg. 45
TDF Analysis (AOAC 985.29)
A TDF analysis directly measures the amount of TDF within a given sample without separately measuring the IDF and SDF
fractions. TDF can be determined using multiple methods. This section describes the procedure for using the ANKOM
TDF
Dietary Fiber Analyzer to determine TDF based on the AOAC 985.29 method.
This analysis requires an SDF filter bag (ANKOM DF-S) for the precipitation process and a non-filter IDF Flow-Thru bag
(ANKOM DF-FT) for the digestion process. When starting a new run, the ANKOM
TDF
Dietary Fiber Analyzer must have bags
installed at all stations (a total of twelve bags).
To perform a TDF analysis, follow the steps below.
1. Prepare chemicals and enzymes.
When using the ANKOM TDF Dietary Fiber Analyzer for the AOAC 985.29 method use the chemicals and enzymes
referred to therein. See Appendix A of this manual for the list of chemicals and enzymes and the instructions for how to
prepare them for use in this instrument.
2. De-fat (see "Appendix C – De-fatting Procedure" in this manual) and de-sugar your samples as
needed according to the AOAC 985.29 method.
3. Label the bags using a Solvent Resistant Marker.
4. Prepare for data collection.
You will need a place to store the data collected during this analysis. For your convenience a CD titled “Calculation
Templates” is included with the instrument. This CD has TDF Data Spreadsheets that can be used for this analysis. Please
read the “Instructions” tab in the MS-Excel file for information about the spreadsheets.
5. Weigh filter bags.
Using a Bag Weigh Holder is critical to eliminate the effects of static electricity
during the weighing process.
Roll or fold each bag and place it in a tared Bag Weigh Holder. Place the Bag Weigh Holder in the center of a balance
and record the weight.
Because different balances have different sensitivities, the Bag Weigh Holder
should be placed in the center of the balance for best results.
To enhance the productivity of your instrument, you can begin the IDF process of a
new run while the SDF process of a previous run is finishing. See the “Productivity
Enhancement” section of this manual for more details.
SDF Bag (longer bag with filter)
IDF Flow-thru Bag (shorter bag / no filter)