ANKOM TDF Dietary - Manual (Page 42)

Operator’s Manual
pg. 42
Rev E 12/2/14
21. Manually measure pH.
One of the questions you are asked before starting the automated procedure is: “Check pH manually?” If you answered
“Yes” to this question, the instrument will stop after adding the required HCl, open Clamp Bar A, display the screen
below, and make a sound to remind you to manually measure pH and adjust if necessary.
When measuring pH use a probe that can be easily rinsed (with Distilled Water) to
avoid loss of sample. If you add acid or base to adjust the pH, you must mix the
solution in order to get an accurate pH reading. To manually mix the solution, press
the outside of the bag with your fingers just above the clamp bar multiple times.
When you have completed the pH measurement process, press the Continue button on the Status screen. You will see
“COMPLETE” next to “pH required?” in the upper left corner of the screen.
22. Make sure that each sample is completely rinsed down into the SDF filter bags (as needed).
Since the IDF Flow-thru bags are not weighed before and after the process, it is important to make sure that each sample
gets rinsed down into the SDF filter bag when the digestion process is complete.
If you notice sample sticking to an IDF Flow-thru bag, use 78% EtOH in a wash bottle to rinse as needed.
23. Rinse the SDF filter bags with acetone.
After the instrument has completed a TDF process the TDF residue that has been collected in the SDF bag must be
manually rinsed with acetone. It is recommended that you use an ANKOM TDF51 Rinse Stand (sold separately) for
rinsing filter bags with acetone.
To rinse the SDF filter bags with acetone using the ANKOM TDF51 Rinse Stand, follow the
steps below.
23.1 Remove the SDF bags from the instrument.
23.2 Place the bags on the Rinse Stand by sliding the back part of the bag under the pinch
mechanism. Keep the top of the bag open.
23.3 Using a wash bottle, squirt acetone into each bag, making sure that all residue on the
clear polypropylene surfaces is rinsed down into the filter.
23.4 Repeat step 23.3 so that each bag gets rinsed a total of two times.
23.5 Allow acetone to evaporate from the bags.
Hazardous Materials – Do NOT heat seal or place bags in an oven until all
acetone has evaporated.
Status
AOAC 991.43 TDF
Overall Progress Action in progress
IDF
Time remaining: Amylase/AMG Phase
XX:XX:XX Time remaining: XX:XX:XX
SDF
Time remaining:
XX:XX:XX
pH required?
Display
Temps/Pressures
YES
IDF
ABORT
PAUSE
SDF
ABORT
Check pH
then press continue
xx:xx:xx
Continue