Operator’s manual – ANKOM TDF Dietary User Manual
Page 57
Operator’s Manual
Rev E 12/2/14
pg. 57
24. Rinse the SDF filter bags with acetone.
After the instrument has completed a TDF process the TDF residue that has been collected in the SDF bag must be
manually rinsed with acetone. It is recommended that you use an ANKOM TDF51 Rinse Stand (sold separately) for
rinsing filter bags with acetone.
To rinse the SDF filter bags with acetone using the ANKOM TDF51 Rinse Stand, follow the steps below.
24.1
Remove the SDF bags from the instrument.
24.2
Place the bags on the Rinse Stand by sliding the back part of the bag under the
pinch mechanism. Keep the top of the bag open.
24.3
Using a wash bottle, squirt acetone into each bag, making sure that all residue on
the clear polypropylene surfaces is rinsed down into the filter.
24.4
Repeat step 24.3 so that each bag gets rinsed a total of two times.
24.5
Allow acetone to evaporate from the bags.
Hazardous Materials
– Do NOT heat seal or place bags in an oven until all
acetone has evaporated.
24.6
With your Heat Sealer set between 3 and 4 (settings may vary depending on
the heat sealer and the power source), seal each bag just above the filter to
keep all residue contained to the filter area while handling the bags.
24.7
Place each bag in the Drying Rack.
25. Dry the SDF filter bags.
25.1
Make sure your oven is 105°C at the location where the bags will be placed.
25.2
Place the Drying Rack with the filter bags in the oven and dry to constant weight (about 90 minutes).
25.3
When dry, remove all SDF filter bags from the oven and place them in a desiccant pouch to cool.
26. Weigh the TDF residue.
26.1
Remove one SDF filter bag from the desiccant pouch.
26.2
Roll or fold the bag, place it in a tared Bag Weigh Holder (BWH), and place the BWH in the center of a balance.
26.3
Record the weight of the filter bag.
26.4
Repeat steps 26.1 – 26.3 for each SDF filter bag used in the fiber analysis.
27. Determine the Protein content within the TDF residue.
See the “Protein Determination” section of this manual for recommended procedures.