HP Data Explorer 4 Series User Manual

Page 184

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Chapter 4 Examining Chromatogram Data

4-12

Applied Biosystems

4

Example

Figure 4-4 shows a TIC that contains three flavonoid
compound peaks. To determine if the diglycosyl group has
fragmented from the parent ion in any of these compounds,
you can generate a CNL extracted chromatogram.

Figure 4-4 TIC for Flavonoid Mixture Containing Three Peaks

Figure 4-5 shows a CNL extracted chromatogram generated
for mass difference of 308.146 m/z which corresponds to the
diglycosyl group.

Figure 4-5 CNL Containing Two Peaks

The CNL extracted chromatogram contains only two peaks,
indicating that the diglycosyl group has fragmented from the
parent ion in the first two compounds only.

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