Creating psd calibration (.cal) files and, Applying to other data files – HP Data Explorer 4 Series User Manual
Page 358
Chapter 8 Viewing Voyager PSD Data
8-20
Applied Biosystems
8
8.3.3 Creating PSD Calibration (.CAL) Files
and Applying to Other Data Files
Creating PSD
.CAL files
To generate a PSD .CAL file:
1.
Acquire a standard, for example, angiotensin, in the
Instrument Control Panel in PSD mode. For more
information, see the Voyager Biospectrometry
Workstation User’s Guide.
2.
Open the .DAT file in the Data Explorer software.
3.
Calibrate as described in Section 8.3.2, Calibrating.
4.
From the File menu, select Export, then select
Calibration.
5.
Name and save the file.
Hint: Include a _PSD suffix when you export a PSD
.CAL file to help you distinguish them from non-PSD
.CAL files. For example, type Cal_PSD as the file
name. The complete file name will be Cal_PSD.CAL.
Applying new
constants to
additional files
You can apply calibration constants from a .CAL file to any
data file. To apply the new constants from a mass calibration
file to a different file:
1.
Display the spectrum to calibrate.
2.
From the Process menu, select Mass Calibration.
3.
Select Import Calibration.
4.
Select the .CAL file to use. Click Open.
The software displays the calibrated spectrum with an
MC trace label.
5.
To save the calibration to the data file, select Mass
Calibration from the Process menu, then select Apply
Calibration.