Chapter 7 data explorer examples, Chapter 8 viewing voyager psd data, Chapter 9 troubleshooting – HP Data Explorer 4 Series User Manual

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Table of Contents

viii

Applied Biosystems

Chapter 7 Data Explorer Examples

7.1

Mariner Data Examples

............................................................

7-2

7.1.1

Improving Signal-To-Noise Ratio ............................................ 7-2

7.1.2

Deconvoluting and Evaluating

Unresolved Chromatographic Peaks .......................................7-4

7.1.3

Determining if a Peak is Background Noise ............................ 7-8

7.2

Voyager Data Examples

..........................................................

7-11

7.2.1

Detecting and Labeling Partially Resolved Peaks .................. 7-11

7.2.2

Processing Before Calibrating to Optimize Mass Accuracy ...7-14

7.2.3

Detecting Peaks from Complex Digests ................................7-18

Chapter 8 Viewing Voyager PSD Data

8.1

Displaying PSD Data

...............................................................

8-2

8.2

Applying Fragment Labels

.........................................................

8-8

8.3

Calibrating a PSD Spectrum

.....................................................

8-10

8.3.1

Checking Peak Detection ...................................................... 8-11

8.3.2

Calibrating ............................................................................8-12

8.3.3

Creating PSD Calibration (.CAL) Files and

Applying to Other Data Files .................................................8-20

8.3.4

Creating PSD Calibration Reference (.REF) Files .................8-21

8.3.5

Changing the Precursor Mass ...............................................8-23

Chapter 9 Troubleshooting

9.1

Overview

..............................................................................

9-2

9.2

General Troubleshooting

..........................................................

9-3

9.3

Processing, Tools, and Applications Troubleshooting

.......................

9-6

9.4

Calibration Troubleshooting

......................................................

9-10

9.5

Printing Troubleshooting

..........................................................

9-14

9.6

Peak Detection and Labeling Troubleshooting

...............................

9-15

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