Luminex xPONENT for MAGPIX User Manual

Page 124

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MagPlex microspheres

Carboxylated spheres with a diameter in the micrometer range. Also
called beads.

median fluorescence
intensity (MFI)

Used to denote how much fluorescence a given particle population
carries; expressed and compared in linear numbers.

microparticle

A solid substance with a diameter in the micrometer range. Often used
as a synonym for a microsphere.

multi-analyte

Several assays or tests performed simultaneously in the same reaction
container.

multi-batch

A set of batches to be processed consecutively.

pending batch

A batch that has been set up, but not yet run.

photobleaching

The process in which light absorption converts the fluorochromes on the
beads into different fluorescent or nonfluorescent compounds.
Photobleaching prevents beads from being properly classified.

plate

A tray in which analytes and samples are placed for organized
acquisition.

plate heater

An aluminum block used in the Luminex analyzer to maintain sample
temperature.

probe

See sample probe.

Qual

A value that determines a cutoff or threshold. This, in conjunction with
ranges using the Lum Qual Formula, Adv Qual Formula, or an edited
range specific for your assay, helps to determine qualitative results for
unknown samples.

qualitative

Pertaining to calculations that determine the absence or presence of an
analyte.

quantitative

Pertaining to calculations that determine the precise numerical
measurement of an analyte.

reagent

A substance used to detect or measure other substances.

reporter

A molecule (or combination of molecules) with a specific range of
excitation and emission wavelengths that is used to identify or quantify
an analyte. Phycoerythrin is the recommended reporter molecule for use
with MAGPIX. Reporter signals are the final result of each assay.

reporter channel

A specific range of wavelengths that includes the emission wavelength of
a designated reporter molecule.

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