Luminex xPONENT for MAGPIX - Manual (Page 66)

• Analysis Type - Use this list to choose from the following analysis types:
• None - No analysis. Select if you have your own data post-processing program and
want to obtain only fluorescent intensity results. You cannot apply standards or controls
when you select None. You cannot analyze acquisitions with this setting.
• Qualitative - Qualitative analysis determines results as either positive or negative,
reactive or non-reactive. The software is flexible in defining custom result ranges, such
as negative, low positive, or high positive. Determinations are based on a single
standard. For qualitative analysis the Luminex software uses a specific algorithm: .
• Quantitative - Determines the sample concentrations from standard curves using
regression method specified in the IVD assay protocol. methods , Linear, Logistic 4P,
and Logistic 5P. Type the desired values for standards and controls in the Number of
Standards and Number of Controls boxes. Select either Fit of All Standards or Mean
of Replicates for the calculation of the curve fit.
• Analyze results while acquiring samples - The software allows real-time viewing of the
results as the instrument analyzes samples. This feature is not available if you select None
as your analysis type.
• Number of Standards - Displays the number of standards for the protocol.
• Use External Analysis Program - This indicates the use of a third-party program to
analyze data.The Analysis Program list becomes active when this is selected. Applies
only to qualitative and quantitative analyses.
• Number of Controls - Displays the number of controls for the protocol.
• Analysis Program - Displays which program is used for data analysis.
• Fit of all Standards - The standard curve will be determined by using each individual
standard replicate when calculating the standard curve. For example, if you run duplicates
of a 7-point standard curve, the software will calculate the standard curve by using 14
points. Applies only to quantitative analysis.
• Mean of Replicates - The standard curve will be determined by averaging the individual
standard replicates when calculating the standard curve. For example, if your un duplicates
of a 7-point standard curve, the software calculates the standard curve by using 14 points.
Applies only to quantitative analysis.
• Cancel - Returns you to the main Batches tab.
Analytes Tab
Use this tab to select or edit analytes used in the batch or protocol.
Using xPONENT
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