Luminex xPONENT for MAGPIX - Manual (Page 18)

normalizes the settings for the classification channels (CL1 and CL2) and the reporter
channel (RP1). Use the Luminex MAGPIX Calibration Kit to accomplish this.
Following calibration, use the Luminex MAGPIX Performance Verification Kit to check all of
the optical channels in the system for correct calibration. It is essential to verify every time
you calibrate. If there is a problem with optical integrity or fluidics, MAGPIX may pass
calibration but fail performance verification. The Luminex MAGPIX Performance Verification
Kit includes reagents to verify the calibration and optical integrity for the Luminex MAGPIX
system as well as reagents to verify the fluidics channels using observations of bead count
and well-to-well carryover.
FIGURE 1. Interrogation of MagPlex ® Beads by the Red and Green LEDs
1. Green LED 2. Camera
3. Chamber 4. Red LED
Repetitive MagPlex Bead Measurements
In a MagPlex assay, the reporter signal is the result of the assay. Due to small bead size,
MagPlex bead suspension exhibits near solution-phase reaction kinetics. This means that
each set of beads used for a particular assay shows a statistically even distribution of
reporter molecules bound to the surface of each bead. The fluorescence signal of reporter
molecules bound to the surface of each bead set is measured and used to determine the
result of each assay in a multiplex. During data acquisition, numerous beads of each set are
analyzed and the median statistic is computed for that set by xPONENT. The more beads of
a set measured, the more confidence that can be given for that particular measurement.
Luminex recommends that you use R-Phycoerythrin as your reporter fluorophore.
Follow the instructions in the IVD kit’s product insert and use the software protocol provided.
Introduction
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